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Plos ONE:中国农大揭miRNA参与玉米应答氮缺乏的调控机制

标签: miRNA
摘要 : 玉米不仅是一种重要的农作物,还是基础博狗官方网站首页学研究的模式物种,氮元素是植物生长必不可少的元素。为了阐明玉米中氮传感与信号传导的分子博狗官方网站首页学机制,近日,中国农大大学李文学研究小组利用miRNA测序对玉米miRNA在氮缺乏条件下的特征展开了研究,相关研究论文发表在Plose One杂志上。
玉米不仅是一种重要的农作物,还是基础博狗官方网站首页学研究的模式物种,氮元素是植物生长必不可少的元素。为了阐明玉米中氮传感与信号传导的分子博狗官方网站首页学机制,近日,中国农大大学李文学研究小组利用miRNA测序对玉米miRNA在氮缺乏条件下的特征展开了研究,相关研究论文发表在Plose One杂志上。 虽然最近研究表明,miRNA能调节植物对于营养缺乏的适应性应答,但对于miRNA在氮缺乏适应性应答的功能仍需进一步探索。 研究人员基于氮缺乏的玉米幼苗样本和对照样本构建了四个小RNA文库,并基于氮缺乏的玉米根样本构建降解组文库。通过深度测序技术,发现了属于47个micrornA家族的共计99个全新的位点,和9个与已知miRNA:miR169,miR171, miR398同源的新位点。Northern blot分析证实8个miRNA家族(5个为保守的,3个为新发现的):miR169s,miR395s,miR528s,miR827s,miRC1,miR169w,miR171p/q,miRC19,以及miRC37在氮缺乏条件下出现显著的下调或上调。分析表明,预测和降解组测序鉴定的新发现miRNAs的靶博狗官方中文德州扑克论坛与广泛的细胞应答与代谢过程密切相关。 玉米作为一种基础博狗官方网站首页学研究的模式物种,此项研究成果也将促进理解miRNA在植物适应氮缺乏中的调控机制。
Plos ONE:中国农大揭miRNA参与玉米应答氮缺乏的调控机制
原文摘要: Cloning and Characterization of Maize miRNAs Involved in Responses to Nitrogen Deficiency Although recent studies indicated that miRNAs regulate plant adaptive responses to nutrient deprivation, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. To elucidate the molecular biology underlying N sensing/signaling in maize, we constructed four small RNA libraries and one degradome from maize seedlings exposed to N deficiency. We discovered a total of 99 absolutely new loci belonging to 47 miRNA families by small RNA deep sequencing and degradome sequencing, as well as 9 new loci were the paralogs of previously reported miR169, miR171, and miR398, significantly expanding the reported 150 high confidence genes within 26 miRNA families in maize. Bioinformatic and subsequent small RNA northern blot analysis identified eight miRNA families (five conserved and three newly identified) differentially expressed under the N-deficient condition. Predicted and degradome-validated targets of the newly identified miRNAs suggest their involvement in a broad range of cellular responses and metabolic processes. Because maize is not only an important crop but is also a genetic model for basic biological research, our research contributes to the understanding of the regulatory roles of miRNAs in plant adaption to N-deficiency stress. 作者:青岚 点击:
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